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| Registros recuperados: 34 | |
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| Madec, Stephanie; Pichereau, Vianney; Jacq, Annick; Paillard, Mathieu; Boisset, Claire; Guerard, Fabienne; Paillard, Christine; Nicolas, Jean-louis. |
| Vibrio tapetis causes the brown ring disease in the Japanese clam Ruditapes philippinarum while Vibrio aestuarianus is associated with massive oyster mortalities. As extracellular proteins are often associated with the virulence of pathogenic bacteria, we undertook a proteomic approach to characterize the secretomes of both vibrios. The extracellular proteins (ECPs) of both species were fractionated by SEC-FPLC and in vitro assays were performed to measure the effects of each fraction on hemocyte cellular parameters (phagocytosis and adhesion). Fractions showing a significant effect were subjected to SDS-PAGE, and proteins were identified by nano LC-MS/MS. 45 proteins were identified for V. aestuarianus and 87 for V. tapetis. Most of them belonged to outer... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00242/35334/34576.pdf |
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| Moussa, M.; Cauvin, E.; Le Piouffle, A.; Lucas, O.; Bidault, Adeline; Paillard, Christine; Benoit, F.; Thuillier, B.; Treilles, M.; Travers, Marie-agnes; Garcia, Celine. |
| In mollusk aquaculture, a large number of Vibrio species are considered major pathogens. Conventional methods based on DNA amplification and sequencing used to accurately identify Vibrio species are unsuitable for monitoring programs because they are time-consuming and expensive. The aim of this study was, therefore, to develop the MALDI-TOF MS method in order to establish a rapid identification technique for a large panel of Vibrio species. We created the EnviBase containing 120 main spectra projections (MSP) of the Vibrio species that are potentially responsible for mollusk diseases, comprising 25 species: V. aestuarianus, V. cortegadensis, V. tapetis and species belonging to the Coralliilyticus, Harveyi, Mediterranei, and Orientalis clades. Each MSP was... |
| Tipo: Text |
Palavras-chave: Vibrio; MALDI-TOF MS; Database; Marine mollusk pathogens. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00680/79224/81732.pdf |
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| Rodrigues, Sophie; Paillard, Christine; Van Dillen, Sabine; Tahrioui, Ali; Berjeaud, Jean-marc; Dufour, Alain; Bazire, Alexis. |
| Marine pathogenic bacteria are able to form biofilms on many surfaces, such as mollusc shells, and they can wait for the appropriate opportunity to induce their virulence. Vibrio tapetis can develop such biofilms on the inner surface of shells of the Ruditapes philippinarum clam, leading to the formation of a brown conchiolin deposit in the form of a ring, hence the name of the disease: Brown Ring Disease. The virulence of V. tapetis is presumed to be related to its capacity to form biofilms, but the link has never been clearly established at the physiological or genetic level. In the present study, we used RNA-seq analysis to identify biofilm- and virulence-related genes displaying altered expression in biofilms compared to the planktonic condition. A... |
| Tipo: Text |
Palavras-chave: Biofilm; Virulence; Vibrio tapetis; Transcriptome; Quorum sensing; Type VI secretion system; Brown ring disease. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00470/58140/60611.pdf |
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| Rahmani, Alexandra; Mathien, Clémentine; Bidault, Adeline; Le Goïc, Nelly; Paillard, Christine; Pichereau, Vianney. |
| Aims Brown Ring Disease (BRD) is an infection of the Manila clam Ruditapes philippinarum due to the pathogen Vibrio tapetis. During BRD, clams are facing immunodepression and shell biomineralization alteration. In this paper, we studied the role of pH on the growth of the pathogen and formulated hypothesis on the establishment of BRD by V. tapetis. Methods and Results In this study, we monitored the evolution of pH during the growth of V. tapetis in a range of pH and temperatures. We also measured the pH of Manila clam hemolymph and extrapallial fluids during infection by V. tapetis. We highlighted that V. tapetis modulates the external pH during its growth, to a value of 7.70. During the development of BRD, V. tapetis also influences extrapallial fluids... |
| Tipo: Text |
Palavras-chave: BRD; Modulation effect; Neutralizing activity; PH; PH modifications; Ruditapes philippinarum; Vibrio tapetis. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00629/74142/73669.pdf |
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| Delavat, Francois; Bidault, Adeline; Pichereau, Vianney; Paillard, Christine. |
| Vibrio campbellii BAA-1116 is renowned for its bioluminescence properties, and genetic tools are available to genetically track this strain. However, many other ecologically important V. harveyi strains exist, for which only few genetic tools are available. In this study, a rapid electroporation protocol was developed to transform replicative plasmids in various environmental V. harveyi and Pseudoalteromonas strains. Moreover, a mini-Tn7 delivery system was modified to site-specifically integrate mini-transposons in the genome of V. harveyi ORM4. As a proof-of-principle, replicative plasmids carrying bioreporters were introduced by electroporation in V. harveyi ORM4 cells, and gene expression was followed at the single cell level. We could demonstrate that... |
| Tipo: Text |
Palavras-chave: Electroporation Mini-transposon Vibrio harveyi Pseudoalteromonas; Phenotypic heterogeneity Bioreporter Flagellum expression. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00459/57035/58934.pdf |
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| Paillard, Christine; Nicolas, Jean-louis; Le Chevalier, Patrick; Lambert, Christophe; Le Boulay, Christine; Berthe, Franck; Haras, Dominique. |
| The emergence of new vibriosis in the European Atlantic coasts has been associated with mass mortalities of molluscs causing important economic losses. For examples, Vibrio carchariae, a bacteria already isolated from shark, has been associated with a severe Haliotis epizooty in hatchery. In oysters, Crass()strea gigas, a strain closely related to Vibrio splendidus is suspected to be associated with summer mortalities. |
| Tipo: Text |
Palavras-chave: Pathogenic vibrios; Bivales; Vibriosis; Bacteriology. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/acte-3287.pdf |
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| Paillard, Christine; Leroux, Frederique; Borrego, Juan. |
| The main microbial diseases affecting marine cultured bivalves have been revised on the basis of the etiologic agents, pathogenesis and pathogenicity. Several recent bivalve-interaction models have been studied, including Pecten larvae-Vibrio pectinicida, brown ring disease, juvenile oyster disease, Pacific oyster nocardiosis and summer mortalities of oysters. In addition, the taxonomy and phylogeny of new potential bivalve pathogens and their virulence factors have been established. Facing the difficulty of identifying bacterial strains associated with molluscan diseases (mainly vibriosis), polyphasic approaches have been developed to correlate the phenotype and genotype of potential pathogens. By evaluating likely virulence mechanisms, developing... |
| Tipo: Text |
Palavras-chave: Diagnostic tools; Virulence factors; Pathogenicity; Pathogenesis; Etiology; Bivalve mollusc; Vibriosis. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-407.pdf |
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| Soletchnik, Patrick; Le Moine, Olivier; Faury, Nicole; Guilpain, Patrice; Geairon, Philippe; Razet, Daniel; Seugnet, Jean-luc; Robert, Stephane; Ropert, Michel; Simonne, Charlotte; Hugonnet, Virginie; Moal, Jeanne; Pouvreau, Stephane; Huvet, Arnaud; Samain, Jean-francois; Lambert, Christophe; Soudant, Philippe; Frouel, Stephane; Choquet, Gwenaelle; Delaporte, Maryse; Paillard, Christine. |
| Four laboratories are involved in this multidisciplinary study: the Conchological Laboratory of Normandie (LCN), the Invertebrates Physiology Laboratory (LPI), the Université Bretagne Occidentale (LEMAR IHP) and the Conchological Laboratory of Poitou-Charentes (LCPC). The part of the study which is presented here (thematics "Coastal ecology") was produced in collaboration with the LCN. The data on the Bay of Veys was collected by Michel Ropert. This study aims at observing the "sites" effect on mortality and on the general biology of the animal, as well as measuring some environmental elements (e.g. water temperature, etc.). The geographical sites are Gefosse in the Bay of Veys (East Cotentin) and the Ronce bank in the south of the Marennes Oléron Bay. The... |
| Tipo: Text |
Palavras-chave: Immunologie; Physiologie; Paramètres environnementaux; Croissance; Ecologie côtière; Baies des Veys Cotentin; Ronce Charente Maritime; Comparaison sites; Reproduction; Mortalités; Crassostrea gigas; Huîtres. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/acte-3405.pdf |
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| Smits, Morgan; Enez, Florian; Ferraresso, Serena; Dalla Rovere, Giulia; Vetois, Emilie; Auvray, Jean-françois; Genestout, Lucie; Mahla, Rachid; Arcangeli, Giuseppe; Paillard, Christine; Haffray, Pierrick; Bargelloni, Luca. |
| The Manila clam Ruditapes philippinarum, a major cultured shellfish species, is threatened by infection with the microparasite Perkinsus olseni, whose prevalence increases with high water temperatures. Under the current trend of climate change, the already severe effects of this parasitic infection might rapidly increase the frequency of mass mortality events. Treating infectious diseases in bivalves is notoriously problematic, therefore selective breeding for resistance represents a key strategy for mitigating the negative impact of pathogens. A crucial step in initiating selective breeding is the estimation of genetic parameters for traits of interest, which relies on the ability to record parentage and accurate phenotypes in a large number of... |
| Tipo: Text |
Palavras-chave: Manila clam (Ruditapes philippinarum); Perkinsus olseni infection; Disease resistance; Genetic selection; Genetic parameter estimation. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00658/77031/78319.pdf |
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| Garcia-bueno, Nuria; Dumay, Justine; Guerin, Thomas; Turpin, Vincent; Paillard, Christine; Stiger-pouvreau, Valerie; Pouchus, Yves-francois; Aitor Marin-atucha, Arnaldo; Decottignies, Priscilla; Fleurence, Joel. |
| The wide polarity range and highly polar compounds of two selected red seaweed, Grateloupia turuturu and Palmaria palmata were extracted using two different types of solvent, dichloromethane/methanol and methanol/water. Monthly in vitro antibacterial activities were studied using the microplate method against the marine bacteria Vibrio harveyi strain ORM4, known to infect abalone. Inhibition, slowdown and delay of Vibrio harveyi growth were investigated. Polar compounds of seaweed showed an activity against the abalone pathogen. The best activity was recorded from P. palmata collected in spring, with an inhibition of 7.9% of the bacterial growth. Preliminary 1H NMR profiles identified the differences between the extracts. |
| Tipo: Text |
Palavras-chave: Antibacterial activity; Abalone disease; Haliotis tuberculata; Red seaweed; Vibrio harveyi. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00617/72875/71929.pdf |
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| Rahmani, Alexandra; Corre, Erwan; Richard, Gaëlle; Bidault, Adeline; Lambert, Christophe; Oliveira, Louisi; Thompson, Cristiane; Thompson, Fabiano; Pichereau, Vianney; Paillard, Christine. |
| The Brown Ring Disease is an infection caused by the bacterium Vibrio tapetis on the Manila clam Ruditapes philippinarum. The process of infection, in the extrapallial fluids (EPFs) of clams, involves alteration of immune functions, in particular on hemocytes which are the cells responsible of phagocytosis. Disorganization of the actin-cytoskeleton in infected clams is a part of what leads to this alteration. This study is the first transcriptomic approach based on collection of extrapallial fluids on living animals experimentally infected by V. tapetis. We performed differential gene expression analysis of EPFs in two experimental treatments (healthy-against infected-clams by V. tapetis), and showed the deregulation of 135 genes. In infected clams, a... |
| Tipo: Text |
Palavras-chave: Brown ring disease; V. tapetis; R. philippinarum; Hemocytes; Actin cytoskeleton; Beta-Thymosin; Coactosin; Resting cells. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00510/62131/66362.pdf |
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| Smits, M.; Artigaud, Sebastien; Bernay, B.; Pichereau, Vianney; Bargelloni, L.; Paillard, Christine. |
| Marine mollusk aquaculture has more than doubled over the past twenty years, accounting for over 15% of total aquaculture production in 2016. Infectious disease is one of the main limiting factors to the development of mollusk aquaculture, and the difficulties inherent to combating pathogens through antibiotic therapies or disinfection have led to extensive research on host defense mechanisms and host-pathogen relationships. It has become increasingly clear that characterizing the functional profiles of response to a disease is an essential step in understanding resistance mechanisms and moving towards more effective disease control. The Manila clam, Ruditapes philippinarum, is a main cultured bivalve species of economic importance which is affected by... |
| Tipo: Text |
Palavras-chave: Bivalve; Diseases; Resistance; Manila clam; Vibrio tapetis; Proteomics; Immune response. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00615/72670/71758.pdf |
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| Arzul, Isabelle; Bass, David; Figueras, Antonio; Lamy, Jean-baptiste; Paillard, Christine; Furones, Dolors. |
| Since 2016, VIVALDI project, supported by the European Union H2020 programme, has aimed to improve the sustainability and competitiveness of the European shellfish industry, which has been hit by a growing number of mortality cases over the recent years. To this end, tools and strategies to better prevent and mitigate the impact of bivalve diseases have been developed through 6 work packages: - In the 1st work package, the diversity and lifecycles of some bivalve pathogens have been investigated. Environmental-based approaches such as passive sensors or the use of magnetic beads and electrochemical biosensors could be useful for pathogen surveillance and the development of early warning systems. - In the 2nd work package, some key mechanisms involved in... |
| Tipo: Text |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00603/71518/69938.pdf |
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| Le Bris, Cedric; Richard, Gaelle; Paillard, Christine; Lambert, Christophe; Seguineau, Catherine; Gauthier, Olivier; Pernet, Fabrice; Guerard, Fabienne. |
| Manila clams, Venerupis philippinarum (Adams and Reeve, 1850), were experimentally challenged with two Vibrio tapetis strains: CECT4600T, the causative agent of Brown Ring Disease (BRD); and LP2 supposedly non-pathogenic in V. philippinarum. Changes in phenoloxidase (PO) and superoxide dismutase (SOD), two major enzymes involved in immunity, were studied in two tissues, the mantle and hemolymph for 30 days after infection in the extrapallial cavity. Bacterial infection in V. philippinarum resulted in modulation of PO and SOD activities that was both tissue- and time-dependent. A response at early times was detected in the mantle and was associated with significant increases in PO and SOD activities in LP2- and CECT4600T-challenged clams 36h post injection.... |
| Tipo: Text |
Palavras-chave: Venerupis philippinarum; Vibrio tapetis; Immunity; Phenoloxidase; Superoxide dismutase. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00221/33257/31733.pdf |
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| Rahmani, Alexandra; Delavat, François; Lambert, Christophe; Le Goic, Nelly; Dabas, Eric; Paillard, Christine; Pichereau, Vianney. |
| Vibrio tapetis is a Gram-negative bacterium that causes infections of mollusk bivalves and fish. The Brown Ring Disease (BRD) is an infection caused by V. tapetis that primarily affects the Manila clam Ruditapes philippinarum. Recent studies have shown that a type IV secretion system (T4SS) gene cluster is exclusively found in strains of V. tapetis pathogenic to clams. However, whether the T4SS is implicated or not during the infection process remains unknown. The aim of this study was to create and characterize a V. tapetis T4SS null mutant, obtained by a near-complete deletion of the virB4 gene, in order to determine the role of T4SS in the development of BRD. This study demonstrated that the T4SS is neither responsible for the loss of hemocyte adhesion... |
| Tipo: Text |
Palavras-chave: Brown Ring Disease; Vibrio tapetis; Ruditapes philippinarum; Hemocytes; Type four secretion system; Rounding phenotype; Colonization; Mutagenesis. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00692/80383/83507.pdf |
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| Schikorski, David; Renault, Tristan; Paillard, Christine; Bidault-toffin, A.; Tourbiez, Delphine; Saulnier, Denis. |
| The Gram-negative bacterium Vibrio harveyi is known to be highly pathogenic for the European abalone Haliotis tuberculata, which is a gastronomically important marine gastropod with a high commercial value. Since 1998, some particular bacterial strains are described as implicated in recurrent mortality outbreaks in French farm and field stocks of abalone. Recently, a 9.6kb plasmid named pVCR1, was shown to be harbored by one highly V. harveyi virulent ORM4 strain suggesting its involvement in virulence phenotype. Thus, we have developed in the present study two TaqMan real-time PCR assays allowing to (i) rapidly and specifically detect, by a duplex procedure and in less than 2 h, both V. harveyi and the presence of plasmid pVCR1 from unidentified bacterial... |
| Tipo: Text |
Palavras-chave: Vibrio harveyi; Haliotis tuberculata; Real-time PCR; Plasmid; Marine pathogen; Molecular diagnostic. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00124/23540/21383.pdf |
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| Trinkler, Nolwenn; Bardeau, Jean-francois; Marin, Frederic; Labonne, Maylis; Jolivet, Aurelie; Crassous, Philippe; Paillard, Christine. |
| The mineral phase of shell repair in the Manila clam Venerupis philippinarum affected by brown ring disease (BRD) was characterised at various scales and at various stages of shell repair by confocal Raman microspectrometry and scanning electron microscopy. Spherulitic and quadrangular aragonite microstructures associated with polyene pigments were clearly observed. Von Kossa staining showed that at the beginning of shell repair, hemocytes are filled with insoluble calcium carbonate salts in all fluids and then are transported toward the extrapallial fluids and the repair sites. Our analyses suggest that after a Vibrio tapetis attack and BRD deposit some clams rapidly cover the deposit, resulting in a modification in the microstructure, which could be... |
| Tipo: Text |
Palavras-chave: Aragonite; Hemocytes; Shell microstructure; Calcium carbonate; Biomineralization; Mollusc; Raman microspectrometry; Scanning electron microscopy. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00029/14065/11301.pdf |
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| De Montaudouin, Xavier; Arzul, Isabelle; Caill-milly, Nathalie; Khayati, Alice; Labrousse, Jean-michel; Lafitte, Céline; Paillard, Christine; Soudant, Philippe; Goulletquer, Philippe. |
| In 1972, France underwent an oyster (Crassostrea angulata) crisis and urgently needed to diversify its aquaculture. Thus, Asari clam (Ruditapes philippinarum) was introduced at that time for aquaculture purpose, concomitantly with the introduction of Pacific oyster (Crassostrea gigas). All Asari clam adults and spat originated from the same area (Puget Sound, WA, USA). After a promising start and the implementation of a national Research & Development program, Asari clam culture rapidly faced a series of concomitant handicaps: firstly, in spite the fact that cultural practices were optimized and locking points addressed, leasing ground availability was limited during the 1980s’ due to certain reluctance from oyster farmers to share their leases and/or... |
| Tipo: Text |
Palavras-chave: Ruditapes philippinarum; France; Aquaculture; Professional fishing; Diseases; Environmental stressors. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00366/47767/47817.pdf |
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| Registros recuperados: 34 | |
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